C (3-6 months) mouse a-CaMKII promoter, Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase DNA, poly A C57BL/6-TgN(a-CaMKII-nlCre)/20 C57BL/6-TgN(a-CaMKII-nlCre)/20 C57BL/6-TgN(a-CaMKII-nlCre)/20 RIKEN,BSI Cre/loxP system 条件を付加する。<br>利用者は寄託者とリソースの利用に関して事前に協議し、承諾書を得る。公表を前提とした学術研究に限る。寄託者との共同研究を基本として利用可能とし、場合により寄託者への謝辞のみで利用可能とする。リソースの論文発表前は共同研究とし、二木啓（Akira Futatsugi）と御子柴克彦（Katsuhiko Mikoshiba）を共同著者として加える。利用者は寄託者の指定論文を引用する。 RBRC00254 <a href='https://brc.riken.jp/mus/pcr00254'>Genotyping protocol -PCR-</a> B6.Cg-Tg(Camk2a-cre)20Kmik/KmikRbrc Developed by Futatsugi A. The Cre-Strain series were transfered to Experimental Animal Division, RIKEN Tsukuba Institute in 1999. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Neurobiology Research The Cre-recombinase activity was detected specifically in the nuclear fraction of the cerebrum. The expression level of the cre in each line of Tg mice was following order; Line 10, 26> 22, 17N > 17A, 11, 20. The expression of the cre was detected by RT-PCR (including semi-quantitativeone), Western blotting and the Cre-recombinase activity assay. There are some variation in the amount of expression in the cerebrum and leaky expression (less than 1/10 to 1/100 of that in the cerebrum at maximum) in the cerebellum among the different lines. Katsuhiko MIKOSHIBA 脳において大脳特異的発現があり、大脳の核分画にCre酵素活性を検出。大脳における発現量はライン10, 26>22, 17N>17A, 11, 20の順。,発現量及び小脳でのleak発現の量 (多くても大脳の1/10〜1/100以下) は系統によりある程度の差がある。 true 理化学研究所脳科学総合研究センター C（3〜6か月） Maintained by sister-brother mating. Breed well, Hemizygote x Wild-type, or hemizygote hemizygote. The Cre-recombinase activity was detected specifically in the nuclear fraction of the cerebrum. The expression level of the cre in each line of Tg mice was following order; Line 10, 26> 22, 17N > 17A, 11, 20. The expression of the cre was detected by RT-PCR (including semi-quantitative one), Western blotting and the Cre-recombinase activity assay. There are some variation in the amount of expression in the cerebrum and leaky expression (less than 1/10 to 1/100 of that in the cerebrum at maximum) in the cerebellum among the different lines. 脳において大脳特異的発現があり、大脳の核分画にCre酵素活性を検出。大脳における発現量はライン10, 26>22, 17N>17A, 11, 20の順。発現量及び小脳でのleak発現の量 (多くても大脳の1/10〜1/100以下) は系統によりある程度の差がある。 御子柴　克彦 The RECIPIENT of BIOLOGICAL RESOURCE shall prior contact with the DEPOSITOR, discussn use of it and obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and NOT for commercial use. The RECIPIENT can use the BIOLOGICAL RESOURCE in a collaboration basis with the DEPOSITOR, except for some cases where only ACKNOLEGEMENT is requested by citing the DEPOSITOR. BEFORE the publication on the BIOLOGICAL RESOURCE by the DEPOSITOR, the RECIPIENT agrees to use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR and add Drs. Akira Futatsugi and Katsuhiko Mikoshiba as a co-authors. The RECIPIENT agrees to cite publications requested by the DEPOSITOR.